Biology lab report on effect of substrate concentration on an enzyme using catechol

Remove the disks, then thoroughly rinse and dry your reaction vessel A. Then, from a molecular perspective the shapes of the graphs can be explained as follows: The same idea applies to the substrate concentration in that the pipettes also had an apparatus error.

However, since the experiment yielded the expected outcomes, it can be considered to have been a successful exercise. The solutions were placed at the test temperature for 5 minutes before mixing the enzyme and substrate, and were kept at the test temperatures when not making readings in the spectrophotometer.

Reaction mixture prepared for each temperature: Be careful not to splash H2O2 on the disks. As the polymers got larger, their colors were seen to deepen from pink-gold through orange-brown and finally to an intense brown-black color.

For example, after setting up the apparatus as described below, you will then measure the amount of O2 collected in the graduated cylinder after one minute. Measure the oxygen level in the graduated cylinder at 1 minute from the time the reaction vessel is turned on its side.

This then prompted the use of interpolation techniques in order to carry out the required analysis. Reaction vessel The following procedures are used to obtain oxygen production and are to be repeated for all Sections A-F.

We set out to measure the rate of the reaction V as a function of temperature. The rate of Melanin production except for the 0. Enzymes catalyze chemical reactions so that they occur in a timely and sequential manner to produce a product.

However, I do not believe the substrate concentrations were significantly different because my repeats were mostly concordant, so a similar amount of oxygen was produced which must mean that there was a similar number of substrate molecules in each concentration.

Your instructor will give you clear directions on how to prepare your complete lab report. The volume of gas in the test tube to start with is slightly affected by the amount which the bung is pushed down each time, if the bung is pushed down further then the volume in the tube will be less so the 30cm3 of gas is reached faster.

You will collect data in terms of the production of one of the products volume of O2 produced in mLand you will then convert to enzyme activity units by dividing the volume of oxygen produced by the amount of time you allow the experiment to run.

The substrate molecules need time to join onto the enzyme and to leave it so the maximum rate achieved is always slightly below the theoretical maximum. This is true because in the reaction, the hydrogen peroxide is observed to have gone through a chemical change; turning into water molecules and dioxide molecules, whereas the liver stayed the same.

We expected the product to develop faster at higher temperature, up to a point. Biology 21 Lab Manual. This would have affected the number of molecules of hydrogen peroxide present, which in turn would have affected the number of collisions between enzyme and substrate molecules.

Actually puzzling usually is best because it means you might actually learn something if you figure it out. The line of best fit also suggests values of concentrations which I have not investigated.

The reaction rates of enzymes were very much affected by changes in temperature, pH and enzyme concentration. Doing this could lead to being disqualified from all the subjects that you are taking.

However, this error was not consistent since the other substrate concentrations followed the expected trend. I did this by only using pipettes, which have a very small apparatus error when compared to beakers.

Enzymes are proteins that speed up the rate of reactions that would otherwise happen more slowly. The solutions were placed at the test temperature for 5 minutes before mixing the enzyme and substrate, and were kept at the test temperatures when not making readings in the spectrophotometer.

Keep the side with the disks upward and carefully place the reaction vessel on its side in the pan of water. The reaction with the highest amounts of the enzyme was the most prolific.

Methods The enzyme solution was approximately 20 grams of potato in 5 L of water 0. In addition, it was observed that the reaction rate for a substrate concentration on 0.

Because the substrate already went through the chemical reaction, it could not go through another one with new catalase, but the old catalase still had functional enzymes and could therefore be reused. Jul 30,  · The variable under study in this experiment was the substrate concentration.

In order to show its effect on enzyme activity, the concentration of the substrate used (catechol) was increased in the following proportions: ml, ml, 1 ml, ml and 3 ml.

With this in mind the following hypothesis was made; as the substrate. This lab covered the measuring of the effects of changes in temperature, pH, and enzyme concentration on reaction rates of an enzyme catalyzed reaction in a controlled experiment. The questions of how environmental factors affect the rate of enzyme-catalyzed reactions were answered in this lab.

Investigation into The Effect Of Substrate Concentration On The Enzyme Catalase.

Investigation: Enzymes

reviewmylife. Tags: A-level, biology, enzyme, experiment, project, substrate concentration. This entry was posted on Thursday, June 5th, at. Data for Enzyme Lab Report. Last updated: This reaction is catalyzed by catechol oxidase, an enzyme present in potato extract.

We expected the product to develop faster at higher temperature, up to a point. 3 ml of enzyme solution and 3 ml of substrate solution.

(You should calculate the concentration of potato and substrate in the. The Enzyme Peroxidase Case Study Lab Report Autumn Vick I.

Effect of Substrate Concentration on the Rate of Activity of Catalase

ABSTRACT: The purpose of this experiment is to learn the effects of a certain enzyme (Peroxidase) concentration, to figure out the temperature and pH effects on Peroxidase activity and the effect of an inhibitor.

thesanfranista.com concentration thesanfranista.comate concentration. thesanfranista.com thesanfranista.com concentration. thesanfranista.comature. thesanfranista.comtor (CuSO 4) Your instructor will give you clear directions on how to prepare your complete lab report. Protocol.

Work as a team of 4 at your table to perform the experiment. Each group will be assigned one or more specific experiments in lab.

Biology lab report on effect of substrate concentration on an enzyme using catechol
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The Effect Of Substrate Concentration On The Activity Of The Enzyme Catalase